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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated MTH1 Monoclonal antibody

MTH1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D7G4

Cat no : CL594-67443

Synonyms

8 oxo dGTPase, MTH1, Nudix motif 1, NUDT1

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-67443

    IF Staining of HeLa using CL594-67443

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 MTH1 antibody (CL594-67443, Clone: 2D7G4 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL594-67443

    FC experiment of Jurkat using CL594-67443

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MTH1 (CL594-67443, Clone:2D7G4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 67443-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-67443 targets MTH1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MTH1 fusion protein Ag9802 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 1
Calculated molecular weight179aa,20 kDa; 197aa,23 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC014618
Gene symbol NUDT1
Gene ID (NCBI) 4521
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTH1, also known as NUDT1 or 8-Oxo-dGTPase, is an important repair enzyme that prevents oxidative stress-induced DNA damage through hydrolyzing mutagenic 8-oxo-dGTP into 8-oxo-dGMP. MTH1 is expressed in postmitotic neurons as well as in proliferative tissues, and it is localized both in the mitochondria and nucleus. MTH1 might be a useful marker of oxidative stress and its level increased upon oxidative stress. Increased expression of MTH1 were also found in various cancer tissues and neurodegenerative diseases.

Protocols

Product Specific Protocols
IF protocol for CL594 MTH1 antibody CL594-67443Download protocol
FC protocol for CL594 MTH1 antibody CL594-67443Download protocol
Standard Protocols
Click here to view our Standard Protocols