• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MSH3 Recombinant antibody

MSH3 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1J23

Cat no : 82845-3-RR

Synonyms

Divergent upstream protein, DUC1, DUG, DUP, hMSH3, Mismatch repair protein 1, MRP1, MSH3, mutS homolog 3 (E. coli)

Validation Data Gallery

Applications:
  • WB analysis using 82845-3-RR

    WB analysis using 82845-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 82845-3-RR

    IF Staining of U-251 using 82845-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHSC-T6 cells
Positive IF detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:750-1:3000
Sample-dependent, check data in validation data gallery

Product Information

82845-3-RR targets MSH3 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MSH3 fusion protein Ag18060 相同性解析による交差性が予測される生物種
Full Name mutS homolog 3 (E. coli)
Calculated molecular weight 1137 aa, 127 kDa
Observed molecular weight 127 kDa
GenBank accession numberBC130434
Gene symbol MSH3
Gene ID (NCBI) 4437
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

Protocols

Product Specific Protocols
WB protocol for MSH3 antibody 82845-3-RRDownload protocol
IF protocol for MSH3 antibody 82845-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols