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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MSH2 Polyclonal antibody

MSH2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15520

Synonyms

COCA1, FCC1, hMSH2, HNPCC, HNPCC1, LCFS2, MSH2, MutS protein homolog 2

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-15520

    FC experiment of HeLa using CL488-15520

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human MSH2 (CL488-15520) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 15520-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-15520 targets MSH2 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MSH2 fusion protein Ag7835 相同性解析による交差性が予測される生物種
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Calculated molecular weight 105 kDa
Observed molecular weight 100 kDa-105 kDa
GenBank accession numberBC021566
Gene symbol MSH2
Gene ID (NCBI) 4436
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 MSH2 antibody CL488-15520Download protocol
Standard Protocols
Click here to view our Standard Protocols