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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPS23 Polyclonal antibody

MRPS23 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18345-1-AP

Synonyms

CGI 138, HSPC329, MRP S23, MRPS23, S23mt

Validation Data Gallery

Applications:
  • WB analysis using 18345-1-AP

    WB analysis using 18345-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18345-1-AP (MRPS23 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 18345-1-AP

    IP experiment of HeLa using 18345-1-AP

    IP result of anti-MRPS23 (IP:18345-1-AP, 4ug; Detection:18345-1-AP 1:300) with HeLa cells lysate 2600ug.

  • IHC staining of human liver cancer using 18345-1-AP

    IHC staining of human liver cancer using 18345-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18345-1-AP (MRPS23 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 18345-1-AP

    IHC staining of human liver cancer using 18345-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18345-1-AP (MRPS23 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 18345-1-AP

    IF Staining of HeLa using 18345-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPS23 antibody (18345-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

18345-1-AP targets MRPS23 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS23 fusion protein Ag13144 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein S23
Calculated molecular weight 22 kDa
Observed molecular weight 23-25 kDa
GenBank accession numberBC000242
Gene symbol MRPS23
Gene ID (NCBI) 51649
RRIDAB_2301078
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MRPS23 antibody 18345-1-APDownload protocol
IHC protocol for MRPS23 antibody 18345-1-APDownload protocol
IF protocol for MRPS23 antibody 18345-1-APDownload protocol
IP protocol for MRPS23 antibody 18345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

Autoregulatory control of mitochondrial glutathione homeostasis

Authors - Yuyang Liu
View Article