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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRPL49 Polyclonal antibody

MRPL49 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15542-1-AP

Synonyms

C11orf4, L49mt, MRP L49, MRPL49, Neighbor of FAU, NOF, NOF1, Protein NOF1

Validation Data Gallery

Applications:
  • WB analysis of BxPC-3 using 15542-1-AP

    WB analysis of BxPC-3 using 15542-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15542-1-AP

    WB analysis of HeLa using 15542-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15542-1-AP

    WB analysis of HeLa using 15542-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 15542-1-AP

    WB analysis of A549 using 15542-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15542-1-AP

    IP experiment of HeLa using 15542-1-AP

    IP result of anti-MRPL49 (IP:15542-1-AP, 4ug; Detection:15542-1-AP 1:500) with HeLa cells lysate 3360ug.

  • IHC staining of human breast cancer using 15542-1-AP

    IHC staining of human breast cancer using 15542-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MRPL49 antibody included
  • IHC staining of human breast cancer using 15542-1-AP

    IHC staining of human breast cancer using 15542-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MRPL49 antibody included

Tested Applications

Positive WB detected inBxPC-3 cells, A549 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MRPL49 Ready-To-Use IHC Kit. MRPL49 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

15542-1-AP targets MRPL49 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPL49 fusion protein Ag7898 相同性解析による交差性が予測される生物種
Full Name mitochondrial ribosomal protein L49
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC004378
Gene symbol MRPL49
Gene ID (NCBI) 740
RRIDAB_2146189
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MRPL49, also named as C11orf4, NOF1, or SW-cl.67, is a 166 amino acid protein, which belongs to the ribosomal protein L49em family. MRPL49 localizes in the Mitochondrion and is widely expressed in various tissues. MRPL49 is a regulator of C3 convertase of classical pathway.

Protocols

Product Specific Protocols
WB protocol for MRPL49 antibody 15542-1-APDownload protocol
IHC protocol for MRPL49 antibody 15542-1-APDownload protocol
IP protocol for MRPL49 antibody 15542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Largen: a molecular regulator of mammalian cell size control.

Authors - Kazuo Yamamoto
View Article
humanWB

Nucleic Acids Res

C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation.

Authors - Wanschers Bas F J BF
View Article
humanWB

Nucleic Acids Res

Replication factors transiently associate with mtDNA at the mitochondrial inner membrane to facilitate replication.

Authors - Nina Rajala
View Article
humanWB

Sci Rep

Human Mitochondrial DNA-Protein Complexes Attach to a Cholesterol-Rich Membrane Structure.

Authors - Joachim M Gerhold
View Article
mouseWB

Cell Rep

MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.

Authors - Jakob D Busch
View Article
humanWB

Nucleic Acids Res

Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB.

Authors - Fenna Hensen
View Article