Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of A2780 using 11706-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11706-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11706-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11706-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11706-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 11706-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 11706-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11706-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 11706-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11706-1-AP (MRPL23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 11706-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11706-1-AP (MRPL23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-3 using 11706-1-AP
Immunofluorescent analysis of PC-3 cells using 11706-1-AP (MRPL23 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 11706-1-AP
Immunofluorescent analysis of MCF-7 cells using 11706-1-AP (MRPL23 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MRPL23 Polyclonal antibody

MRPL23 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11706-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MRP-L23, MRP L23, Large ribosomal subunit protein uL23m, L23mt, L23MRP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A2780 cells, HEK-293 cells, HeLa cells, MCF-7 cells, PC-3 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-3 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below

Product Information

11706-1-AP targets MRPL23 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2220 Product name: Recombinant human MRPL23 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-153 aa of BC027710 Sequence: MARNVVYPLYRLGGPQLRVFRTNFFIQLVRPGVAQPEDTVQFRIPMEMTRVDLRNYLEGIYNVPVAAVRTRVQHGSNKRRDHRNVRIKKPDYKVAYVQLAHGQTFTFPDLFPEKDESPEGSAADDLYSMLEEERQQRQSSDPRRGGVPSWFGL 相同性解析による交差性が予測される生物種
Full Name	mitochondrial ribosomal protein L23
Calculated molecular weight	153 aa, 18 kDa
Observed molecular weight	18 kDa
GenBank accession number	BC027710
Gene Symbol	MRPL23
Gene ID (NCBI)	6150
RRID	AB_10694422
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16540
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for MRPL23 antibody 11706-1-AP	Download protocol
IHC protocol for MRPL23 antibody 11706-1-AP	Download protocol
WB protocol for MRPL23 antibody 11706-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice. Authors - Tara R Richman View Article
human	WB	Nucleic Acids Res METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis. Authors - Lindsey Van Haute View Article
mouse	WB	Aging Cell Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan. Authors - Tara R Richman View Article
mouse	WB	J Cell Biol Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness. Authors - Uwe Richter View Article
mouse	WB	PLoS Genet Mutation in MRPS34 Compromises Protein Synthesis and Causes Mitochondrial Dysfunction. Authors - Tara R Richman View Article
human	WB	Genomics Proteomics Bioinformatics Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses. Authors - Ruona Shi View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A2780 using 11706-1-AP

WB analysis of MCF-7 using 11706-1-AP

WB analysis of HEK-293 using 11706-1-AP

WB analysis of HEK-293 using 11706-1-AP

WB analysis of HeLa using 11706-1-AP

WB analysis of A2780 using 11706-1-AP

WB analysis of PC-3 using 11706-1-AP

WB analysis of MCF-7 using 11706-1-AP

IHC staining of human liver cancer using 11706-1-AP

IHC staining of human liver cancer using 11706-1-AP

IF Staining of PC-3 using 11706-1-AP

IF Staining of MCF-7 using 11706-1-AP

MRPL23 Polyclonal antibody

MRPL23 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.

Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.

Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.

Mutation in MRPS34 Compromises Protein Synthesis and Causes Mitochondrial Dysfunction.

Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.