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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MRP1 Polyclonal antibody

MRP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27825-1-AP

Synonyms

ABC29, ABCC, ABCC1, GS X, Leukotriene C(4) transporter, LTC4 transporter, MRP, MRP1

Validation Data Gallery

Applications:
  • WB analysis using 27825-1-AP

    WB analysis using 27825-1-AP

    37°C incubated HeLa cells were subjected to SDS PAGE followed by western blot with 27825-1-AP (MRP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27825-1-AP

    IHC staining of human breast cancer using 27825-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27825-1-AP (ABCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 27825-1-AP

    IHC staining of human breast cancer using 27825-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27825-1-AP (ABCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected in37°C incubated HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27825-1-AP targets MRP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRP1 fusion protein Ag27048 相同性解析による交差性が予測される生物種
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 1
Calculated molecular weight 172 kDa
Observed molecular weight 180-190 kDa
GenBank accession numberNM_004996
Gene symbol ABCC1
Gene ID (NCBI) 4363
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Multidrug resistant-associated protein 1 (MRP1, also known as ABCC1) is a member of the ATP-binding cassette (ABC) transporter protein superfamily, subfamily C. MRP1 is overexpressed in cancers and contributes to the occurrence of multidrug resistance (MDR). Expression of MRP1 has been considered as a negative marker for the outcome of chemotherapy. Recently MRP1 has been reported to play a crucial role in Aβ clearance at the blood-brain barrier.

Protocols

Product Specific Protocols
WB protocol for MRP1 antibody 27825-1-APDownload protocol
IHC protocol for MRP1 antibody 27825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols