Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10370-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10370-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 10370-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 10370-1-AP
IP result of anti-MOV10 (IP:10370-1-AP, 4ug; Detection:10370-1-AP 1:5000) with HepG2 cells lysate 1480 ug.
IHC staining of human prostate hyperplasia using 10370-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10370-1-AP (MOV10 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human prostate hyperplasia using 10370-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10370-1-AP (MOV10 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HepG2 using 10370-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MOV10 antibody (10370-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of MCF-7 using 10370-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MOV10 antibody (10370-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

MOV10 Polyclonal antibody

MOV10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10370-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

Armitage homolog, EC:3.6.4.13, fSAP113, gb110, Helicase MOV-10

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (28)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, human liver tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human prostate hyperplasia tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 23 publications below
IHC	See 1 publications below
IF	See 8 publications below
IP	See 4 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

10370-1-AP targets MOV10 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0384 Product name: Recombinant human MOV10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 869-1003 aa of BC002548 Sequence: QGQERSVILISTVRSSQSFVQLDLDFNLGFLKNPKRFNVAVTRAKALLIIVGNPLLLGHDPDWKVFLEFCKENGGYTGCPFPAKLDLQQGQNLLQGLSKLSPSTSGPHSHDYLPQEREGEGGLSLQVEPEWRNEL 相同性解析による交差性が予測される生物種
Full Name	Mov10, Moloney leukemia virus 10, homolog (mouse)
Calculated molecular weight	114 kDa
Observed molecular weight	110-115 kDa
GenBank accession number	BC002548
Gene Symbol	MOV10
Gene ID (NCBI)	4343
RRID	AB_2297897
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9HCE1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

Protocols

Product Specific Protocols
IF protocol for MOV10 antibody 10370-1-AP	Download protocol
IHC protocol for MOV10 antibody 10370-1-AP	Download protocol
IP protocol for MOV10 antibody 10370-1-AP	Download protocol
WB protocol for MOV10 antibody 10370-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Neuron A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation. Authors - Banerjee Sourav S View Article
		Mol Cell Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change. Authors - Ameya P Jalihal View Article
human	WB	Mol Cell MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs. Authors - Lea H Gregersen KD Validated View Article
human	WB,IP	Nat Struct Mol Biol Capped small RNAs and MOV10 in human hepatitis delta virus replication. Authors - Haussecker Dirk D KD Validated View Article
human	WB,IF	PLoS Genet MOV10 RNA helicase is a potent inhibitor of retrotransposition in cells. Authors - Goodier John L JL View Article
human	IF	PLoS Pathog APOBEC3G and APOBEC3F require an endogenous cofactor to block HIV-1 replication. Authors - Han Yanxing Y View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10370-1-AP

WB analysis of HepG2 using 10370-1-AP

WB analysis of human liver using 10370-1-AP

IP experiment of HepG2 using 10370-1-AP

IHC staining of human prostate hyperplasia using 10370-1-AP

IHC staining of human prostate hyperplasia using 10370-1-AP

IF Staining of HepG2 using 10370-1-AP

IF Staining of MCF-7 using 10370-1-AP

MOV10 Polyclonal antibody

MOV10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation.

Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.

MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs.

Capped small RNAs and MOV10 in human hepatitis delta virus replication.

MOV10 RNA helicase is a potent inhibitor of retrotransposition in cells.

APOBEC3G and APOBEC3F require an endogenous cofactor to block HIV-1 replication.