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  • Featured Product
  • KD/KO Validated

MON1B Monoclonal antibody

MON1B Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3E7C6

Cat no : 67985-1-Ig

Synonyms

HSRG1, KIAA0872, MON1 homolog B (yeast), MON1B, SAND2

Validation Data Gallery

Applications:
  • WB analysis using 67985-1-Ig

    WB analysis using 67985-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67985-1-Ig (MON1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of A549 using 67985-1-Ig

    WB analysis of A549 using 67985-1-Ig

    WB result of MON1B antibody (67985-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected A549 cells.

  • WB analysis of HeLa using 67985-1-Ig

    WB analysis of HeLa using 67985-1-Ig

    WB result of MON1B antibody (67985-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected HeLa cells.

  • IF Staining of HeLa using 67985-1-Ig

    IF Staining of HeLa using 67985-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MON1B antibody (67985-1-Ig, Clone: 3E7C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HepG2 cells, K-562 cells, SW480 cells, MCF-7 cells
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67985-1-Ig targets MON1B in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MON1B fusion protein Ag9920 相同性解析による交差性が予測される生物種
Full Name MON1 homolog B (yeast)
Calculated molecular weight 547 aa, 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC024277
Gene symbol MON1B
Gene ID (NCBI) 22879
RRIDAB_2918734
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vacuolar fusion protein MON1 homolog B (MON1B) is also named as HSRG1, KIAA0872 and SAND2, and belongs to the MON1/SAND family. The membrane recruitment of MON1B, a homolog of yeast MON1 protein in mammals, is essential for vesicular fusion of early endosomes (PMID:26987402).

Protocols

Product Specific Protocols
WB protocol for MON1B antibody 67985-1-IgDownload protocol
IF protocol for MON1B antibody 67985-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols