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  • Featured Product
  • KD/KO Validated

MMP3 Polyclonal antibody

MMP3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17873-1-AP

Synonyms

CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1

Validation Data Gallery

Applications:
  • WB analysis using 17873-1-AP

    WB analysis using 17873-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17873-1-AP

    WB analysis of HeLa using 17873-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human skeletal muscle using 17873-1-AP

    WB analysis of human skeletal muscle using 17873-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17873-1-AP

    WB analysis of HeLa using 17873-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 17873-1-AP

    WB analysis of A549 using 17873-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A2780 using 17873-1-AP

    WB analysis of A2780 using 17873-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 17873-1-AP

    IHC staining of human breast cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with MMP3 antibody included
  • IHC staining of human breast cancer using 17873-1-AP

    IHC staining of human breast cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with MMP3 antibody included
  • IHC staining of human stomach cancer using 17873-1-AP

    IHC staining of human stomach cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MMP3 antibody included
  • IHC staining of human stomach cancer using 17873-1-AP

    IHC staining of human stomach cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MMP3 antibody included
  • IHC staining of human breast cancer using 17873-1-AP

    IHC staining of human breast cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MMP3 antibody included
  • IHC staining of human breast cancer using 17873-1-AP

    IHC staining of human breast cancer using 17873-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MMP3 antibody included
  • IF Staining of A549 using 17873-1-AP

    IF Staining of A549 using 17873-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP3 antibody (17873-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A549 using 17873-1-AP

    IF Staining of A549 using 17873-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP3 antibody (17873-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

Tested Applications

Positive WB detected inA2780 cells, A549 cells, HeLa cells, human skeletal muscle tissue, U-87 MG cells, PC-12 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy MMP3 Ready-To-Use IHC Kit. MMP3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

17873-1-AP targets MMP3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse, rabbit, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MMP3 fusion protein Ag12359 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Calculated molecular weight 477 aa, 54 kDa
Observed molecular weight 45-60 kDa
GenBank accession numberBC074869
Gene symbol MMP3
Gene ID (NCBI) 4314
RRIDAB_2146587
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.

Protocols

Product Specific Protocols
WB protocol for MMP3 antibody 17873-1-APDownload protocol
IHC protocol for MMP3 antibody 17873-1-APDownload protocol
IF protocol for MMP3 antibody 17873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB,IF

Autophagy

Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration

Authors - Di Wang
View Article
humanIHC

Bone Res

FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis.

Authors - Dong Guo
View Article
humanWB

Mater Today Bio

Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction

Authors - Zhanqiu Dai
View Article
ratWB

Phytomedicine

Systemic pharmacology reveal the mechanism by which the Qiangjin Zhuanggu Qufeng mixture inhibits LPS-induced pyroptosis of rat nucleus pulposus cells

Authors - Dong Wang
View Article
humanWB

Clin Cancer Res

BATF2 Deficiency Promotes Progression in Human Colorectal Cancer via Activation of HGF/MET Signaling: A Potential Rationale for Combining MET Inhibitors with IFNs.

Authors - Zebing Liu
View Article
mouse

Gut Microbes

Gut microbiota regulate tumor metastasis via circRNA/miRNA networks.

Authors - Zhuxian Zhu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ana Luísa (Verified Customer) (07-25-2022)

Permeabilization: 0,3% Triton X-100 Blocking 2,5% BSA + 0,1% Triton X-100

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Intervertebral disc cells