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Target

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Species Reactivity

Species Reactivity

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Conjugate

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated MMP2 Monoclonal antibody

MMP2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, mouse, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B10D1

Cat no : CL488-66366

Synonyms

72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MONA, TBE 1

Validation Data Gallery

Applications:
  • IF Staining of MCF-7 using CL488-66366

    IF Staining of MCF-7 using CL488-66366

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®®488 MMP2 antibody (CL488-66366, Clone: 2B10D1 ) at dilution of 1:200.

Tested Applications

Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MMP2 Ready-To-Use IHC Kit. MMP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66366-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66366 targets MMP2 in IF applications and shows reactivity with human, rat, mouse, pig samples.

Tested Reactivity human, rat, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MMP2 fusion protein Ag25039 相同性解析による交差性が予測される生物種
Full Name matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
Calculated molecular weight 72 kDa
Observed molecular weight 55-74 kDa
GenBank accession numberBC002576
Gene symbol MMP2
Gene ID (NCBI) 4313
RRIDAB_2919317
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

MMP2, also named as CLG4A, Gelatinase Am, TBE-1 and PEX, belongs to the peptidase M10A family. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro. MMP2 can be cleaved into PEX chain(~60kd).Western blot analysis showed that the 72 kDa and 62 kDa proteinase activities were pro-MMP2 and the active enzyme,respectively(PMID:11112697).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 MMP2 antibody CL488-66366Download protocol
Standard Protocols
Click here to view our Standard Protocols