Validation Data Gallery
Filter:
This product is currently not available for sale.
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66349 targets MKP-2 in IF applications and shows reactivity with human, pig, rat, mouse samples.
| Tested Reactivity | human, pig, rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MKP-2 fusion protein Ag1206 相同性解析による交差性が予測される生物種 |
| Full Name | dual specificity phosphatase 4 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 43 kDa, 33 kDa |
| GenBank accession number | BC014565 |
| Gene Symbol | DUSP4 |
| Gene ID (NCBI) | 1846 |
| RRID | AB_2883315 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13115 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 MKP-2 antibody CL488-66349 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |