MIP-3 Alpha Polyclonal antibody

MIP-3 Alpha Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26527-1-AP

Synonyms

Beta chemokine exodus 1, C C motif chemokine 20, CC chemokine LARC, CCL20, CKb4, LARC, MIP 3 alpha, MIP 3a, MIP3A, MIP-3α, SCYA20, Small inducible cytokine A20, ST38



Tested Applications

Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CCL20/MIP3A Ready-To-Use IHC Kit. CCL20/MIP3A primary antibody included.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26527-1-AP is MIP-3 Alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIP-3 Alpha fusion protein Ag24069 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 20
Calculated molecular weight 11 kDa
GenBank accession numberBC020698
Gene symbol CCL20
Gene ID (NCBI) 6364
RRIDAB_2880543
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.

Protocols

Product Specific Protocols
IHC protocol for MIP-3 Alpha antibody 26527-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Gut Microbes

Fusobacterium nucleatum promotes colorectal cancer metastasis through miR-1322/CCL20 axis and M2 polarization

Authors - Chaochao Xu
humanIF

Front Cell Dev Biol

EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma.

Authors - Bin Qi
mouse

Phytomedicine

The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides.

Authors - Gaofeng Cai
humanIHC

Oncol Rep

DEPDC1 drives hepatocellular carcinoma cell proliferation, invasion and angiogenesis by regulating the CCL20/CCR6 signaling pathway.

Authors - Wubin Guo
humanIHC

Inflammation

PMID: 31838663

Authors - YingYing Hu
humanIHC

Oncol Lett

Overexpression of B7-H4 promotes renal cell carcinoma progression by recruiting tumor-associated neutrophils via upregulation of CXCL8.

Authors - Anqi Li