Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

U-937 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Y79 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-MIF (IP:20415-1-AP, 4ug; Detection:20415-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MIF antibody (20415-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human MIF (20415-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.