Validation Data Gallery
Tested Applications
| Positive FC detected in | BALB/c mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.125 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 2 publications below |
Product Information
APC-65122 targets MHC Class II (I-A/I-E) in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Activated C57BL/6 mouse spleen cells 相同性解析による交差性が予測される生物種 |
| Full Name | histocompatibility 2, class II antigen A, alpha |
| GenBank accession number | BC031711 |
| Gene Symbol | MHC Class II |
| Gene ID (NCBI) | 14960 |
| RRID | AB_2883008 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 650 nm / 660 nm |
| Form | |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P14434 |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.2 |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC MHC Class II (I-A/I-E) antibody APC-65122 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Ophthalmol Do the monocyte-derived dendritic cells exert a pivotal role in the early onset of experimental autoimmune uveitis | ||
Nutrients The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype |
