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  • KD/KO Validated

RNF156 Polyclonal antibody

RNF156 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11285-1-AP

Synonyms

KIAA0544, mahogunin, ring finger 1, MGRN1, RING finger protein 156, RNF156



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11285-1-AP targets RNF156 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF156 fusion protein Ag1808 相同性解析による交差性が予測される生物種
Full Name mahogunin, ring finger 1
Calculated molecular weight 63 kDa
Observed molecular weight 58-64 kDa
GenBank accession numberBC014199
Gene symbol MGRN1
Gene ID (NCBI) 23295
RRIDAB_2143351
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MGRN1(E3 ubiquitin-protein ligase) mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. It also plays a role in the regulation of endosome-to-lysosome trafficking. Human and mouse mahogunin exhibit a similar expression pattern across multiple tissues with particularly high levels in brain(PMID: 12560552). It has 4 isoforms produced by alternative splicing with the molecular mass of 58-64 kDa.

Protocols

Product Specific Protocols
WB protocol for RNF156 antibody 11285-1-APDownload protocol
IHC protocol for RNF156 antibody 11285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Cell

Aging Triggers Cytoplasmic Depletion and Nuclear Translocation of the E3 Ligase Mahogunin: A Function for Ubiquitin in Neuronal Survival.

Authors - Stefano Benvegnù
  • KD Validated
humanWB

Dev Cell

A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development.

Authors - Jennifer H Kong
humanWB

Pigment Cell Melanoma Res

MGRN1-dependent pigment-type switching requires its ubiquitination activity but not its interaction with TSG101 or NEDD4.

Authors - Gunn Teresa M TM
human,mouseIP

Development

Gene-teratogen interactions influence the penetrance of birth defects by altering Hedgehog signaling strength.

Authors - Jennifer H Kong
humanWB,IHC

Front Oncol

Promoter Methylation of the MGRN1 Gene Predicts Prognosis and Response to Chemotherapy of High-Grade Serous Ovarian Cancer Patients.

Authors - Xiao-Fei Li
mouseWB,IF

J Biol Chem

Mahoganoid and mahogany mutations rectify the obesity of the yellow mouse by effects on endosomal traffic of MC4R protein.

Authors - Overton John D JD
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Parijat (Verified Customer) (09-20-2023)

Works well.

  • Applications: Western Blot