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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MFAP4 Polyclonal antibody

MFAP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17661-1-AP

Synonyms

MFAP4

Validation Data Gallery

Applications:
  • WB analysis of human kidney using 17661-1-AP

    WB analysis of human kidney using 17661-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of rat lung using 17661-1-AP

    WB analysis of rat lung using 17661-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • WB analysis of mouse heart using 17661-1-AP

    WB analysis of mouse heart using 17661-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 17661-1-AP

    WB analysis of mouse lung using 17661-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 17661-1-AP

    IHC staining of human ovary using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human ovary using 17661-1-AP

    IHC staining of human ovary using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human placenta using 17661-1-AP

    IHC staining of human placenta using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human placenta using 17661-1-AP

    IHC staining of human placenta using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human testis using 17661-1-AP

    IHC staining of human testis using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 17661-1-AP

    IHC staining of human testis using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human skin using 17661-1-AP

    IHC staining of human skin using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 17661-1-AP

    IHC staining of human skin using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human spleen using 17661-1-AP

    IHC staining of human spleen using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 17661-1-AP

    IHC staining of human spleen using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human lung using 17661-1-AP

    IHC staining of human lung using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 17661-1-AP

    IHC staining of human lung using 17661-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inhuman kidney tissue, mouse heart tissue, mouse lung tissue, rat lung tissue
Positive IHC detected inhuman ovary tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17661-1-AP targets MFAP4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFAP4 fusion protein Ag11920 相同性解析による交差性が予測される生物種
Full Name microfibrillar-associated protein 4
Calculated molecular weight 255 aa, 29 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC062415
Gene symbol MFAP4
Gene ID (NCBI) 4239
RRIDAB_2142355
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)

Protocols

Product Specific Protocols
WB protocol for MFAP4 antibody 17661-1-APDownload protocol
IHC protocol for MFAP4 antibody 17661-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Thorax

A large lung gene expression study identifying fibulin-5 as a novel player in tissue repair in COPD.

Authors - Corry-Anke Brandsma
View Article
humanWB

J Proteome Res

Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples.

Authors - Muraoka Satoshi S
View Article
humanIHC, IF

FASEB J

The "Matrisome" reveals the characterization of skin keloid microenvironment.

Authors - Shikun Zhang
View Article
mouseWB

J Proteomics

FOXL1 + Telocytes in mouse colon orchestrate extracellular matrix biodynamics and wound repair resolution

Authors - Veronique Pomerleau
View Article