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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL3 Monoclonal antibody (67733-2-Ig)

    WB analysis using 67733-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67733-2-Ig (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using METTL3 Monoclonal antibody (67733-2-Ig)

    WB analysis of HeLa using 67733-2-Ig

    WB result of METTL3 antibody (67733-2-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using METTL3 Monoclonal antibody (67733-2-Ig)

    IHC staining of human lung cancer using 67733-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using METTL3 Monoclonal antibody (67733-2-Ig)

    IHC staining of human urothelial carcinoma using 67733-2-Ig

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using METTL3 Monoclonal antibody (67733-2-Ig)

    IHC staining of human urothelial carcinoma using 67733-2-Ig

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using METTL3 Monoclonal antibody (67733-2-Ig)

    IHC staining of mouse testis using 67733-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using METTL3 Monoclonal antibody (67733-2-Ig)

    IHC staining of mouse testis using 67733-2-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using METTL3 Monoclonal antibody (67733-2-Ig)

    IHC staining of human lung cancer using 67733-2-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67733-2-Ig (METTL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

METTL3 Monoclonal antibody

METTL3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67733-2-Ig
Clone No.4B4A3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Neuro-2a cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human urothelial carcinoma tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67733-2-Ig targets METTL3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7110

Product name: Recombinant human METTL3 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 229-580 aa of BC001650

Sequence: LNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRSRGRAQVQEFCDYGTKEECMKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHKPDEIYGMIERLSPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQRYPDGIISKPKNL

相同性解析による交差性が予測される生物種
Full Name methyltransferase like 3
Calculated molecular weight 64 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC001650
Gene Symbol METTL3
Gene ID (NCBI) 56339
RRIDAB_2918503
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ86U44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for METTL3 antibody 67733-2-IgDownload protocol
IHC protocol for METTL3 antibody 67733-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols