Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse colon tissue, mouse brain tissue, mouse heart tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 31 publications below |
| IHC | See 11 publications below |
| IF | See 17 publications below |
| IP | See 1 publications below |
| ChIP | See 2 publications below |
Product Information
10056-1-AP targets MEF2C in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0020 Product name: Recombinant human MEF2C protein Source: e coli.-derived, Unknow Tag: N/A Domain: MEF2C of BC156603 Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | myocyte enhancer factor 2C |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 45-70 kDa |
| GenBank accession number | BC156603 |
| Gene Symbol | MEF2C |
| Gene ID (NCBI) | 4208 |
| RRID | AB_513447 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06413 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MEF2C antibody 10056-1-AP | Download protocol |
| IF protocol for MEF2C antibody 10056-1-AP | Download protocol |
| IHC protocol for MEF2C antibody 10056-1-AP | Download protocol |
| IP protocol for MEF2C antibody 10056-1-AP | Download protocol |
| WB protocol for MEF2C antibody 10056-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Biotechnol Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. | ||
Cell Res Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans. | ||
Nat Neurosci Tbr1 haploinsufficiency impairs amygdalar axonal projections and results in cognitive abnormality. | ||
Nat Commun NitroSynapsin therapy for a mouse MEF2C haploinsufficiency model of human autism. | ||
Genes Dev Gsx2 controls region-specific activation of neural stem cells and injury-induced neurogenesis in the adult subventricular zone. |
