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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated MED30 Monoclonal antibody

MED30 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D7C4

Cat no : CL488-67038

Synonyms

MED30, mediator complex subunit 30, THRAP6, TRAP25

Validation Data Gallery

Applications:
  • FC experiment of HeLa using CL488-67038

    FC experiment of HeLa using CL488-67038

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MED30 (CL488-67038, Clone:1D7C4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67038-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67038 targets MED30 in FC (Intra) applications and shows reactivity with Human, rat, pig samples.

Tested Reactivity Human, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MED30 fusion protein Ag10235 相同性解析による交差性が予測される生物種
Full Name mediator complex subunit 30
Calculated molecular weight 178 aa, 20 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC008226
Gene symbol MED30
Gene ID (NCBI) 90390
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MED30, also named as THRAP6 and TRAP25, is a component of the Mediator complex. MED30 has two isoforms with MW 16-20 kDa ad 20-25 kDa.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 MED30 antibody CL488-67038Download protocol
Standard Protocols
Click here to view our Standard Protocols