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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated MCM7 Monoclonal antibody

MCM7 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G1F6

Cat no : CL594-67446

Synonyms

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-67446

    IF Staining of HepG2 using CL594-67446

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 MCM7 antibody (CL594-67446, Clone: 1G1F6 ) at dilution of 1:200, CL488-Phalloidin (green).

Tested Applications

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MCM7 Ready-To-Use IHC Kit. MCM7 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67446-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67446 targets MCM7 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCM7 fusion protein Ag28558 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 7
Calculated molecular weight 81 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC013375
Gene symbol MCM7
Gene ID (NCBI) 4176
RRIDAB_2920137
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2-7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].

Protocols

Product Specific Protocols
IF protocol for CL594 MCM7 antibody CL594-67446Download protocol
Standard Protocols
Click here to view our Standard Protocols