• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

MCM6 Polyclonal antibody

MCM6 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13347-2-AP

Synonyms

MCG40308, MCM6, Mis5, P105MCM

Validation Data Gallery

Applications:
  • WB analysis using 13347-2-AP

    WB analysis using 13347-2-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 13347-2-AP

    WB analysis of HeLa using 13347-2-AP

    WB result of MCM6 antibody (13347-2-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM6 transfected HeLa cells.

  • WB analysis of mouse ovary using 13347-2-AP

    WB analysis of mouse ovary using 13347-2-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse thymus using 13347-2-AP

    WB analysis of mouse thymus using 13347-2-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse spleen using 13347-2-AP

    WB analysis of mouse spleen using 13347-2-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13347-2-AP (MCM6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 13347-2-AP

    IP experiment of HeLa using 13347-2-AP

    IP result of anti-MCM6 (IP:13347-2-AP, 3ug; Detection:13347-2-AP 1:1000) with HeLa cells lysate 3800ug.

  • IHC staining of human cervical cancer using 13347-2-AP

    IHC staining of human cervical cancer using 13347-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13347-2-AP (MCM6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IHC staining of human lung cancer using 13347-2-AP

    IHC staining of human lung cancer using 13347-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13347-2-AP (MCM6 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IHC staining of human lung cancer using 13347-2-AP

    IHC staining of human lung cancer using 13347-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13347-2-AP (MCM6 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IHC staining of human cervical cancer using 13347-2-AP

    IHC staining of human cervical cancer using 13347-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IHC staining of human cervical cancer using 13347-2-AP

    IHC staining of human cervical cancer using 13347-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13347-2-AP (MCM6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IHC staining of human cervical cancer using 13347-2-AP

    IHC staining of human cervical cancer using 13347-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IHC staining of human cervical cancer using 13347-2-AP

    IHC staining of human cervical cancer using 13347-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IHC staining of human cervical cancer using 13347-2-AP

    IHC staining of human cervical cancer using 13347-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IHC staining of human cervical cancer using 13347-2-AP

    IHC staining of human cervical cancer using 13347-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IHC staining of human cervical cancer using 13347-2-AP

    IHC staining of human cervical cancer using 13347-2-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13347-2-AP (MCM6 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with MCM6 antibody included
  • IF Staining of HeLa using 13347-2-AP

    IF Staining of HeLa using 13347-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM6 antibody (13347-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, mouse ovary tissue, mouse thymus tissue, mouse spleen tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MCM6 Ready-To-Use IHC Kit. MCM6 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

13347-2-AP targets MCM6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCM6 fusion protein Ag4180 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 6
Calculated molecular weight 821 aa, 93 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC032374
Gene symbol MCM6
Gene ID (NCBI) 4175
RRIDAB_2142537
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.

Protocols

Product Specific Protocols
WB protocol for MCM6 antibody 13347-2-APDownload protocol
IHC protocol for MCM6 antibody 13347-2-APDownload protocol
IF protocol for MCM6 antibody 13347-2-APDownload protocol
IP protocol for MCM6 antibody 13347-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Hematol Oncol

Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma

Authors - Yexin Tong
View Article
humanWB

Biomaterials

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Authors - Hongyuan Song
View Article
humanWB

Elife

MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.

Authors - Yuichiro Saito
View Article
humanWB

Front Oncol

METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway.

Authors - Dong-Dong Yang
View Article
humanWB

Oncotarget

Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.

Authors - Yinan Ma
View Article
humanWB

Ecotoxicol Environ Saf

The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.

Authors - Zemin Li
View Article