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  • Featured Product
  • KD/KO Validated

MCM2 Monoclonal antibody

MCM2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F4D10

Cat no : 66204-1-Ig

Synonyms

BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 66204-1-Ig

    WB analysis of HEK-293 using 66204-1-Ig

    WB result of MCM2 antibody (66204-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells.

  • WB analysis using 66204-1-Ig

    WB analysis using 66204-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 66204-1-Ig

    WB analysis of 4T1 using 66204-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 66204-1-Ig

    WB analysis of K-562 using 66204-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66204-1-Ig

    WB analysis of HEK-293 using 66204-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 66204-1-Ig

    WB analysis of A431 using 66204-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66204-1-Ig

    IHC staining of human prostate cancer using 66204-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM2 antibody included
  • IHC staining of human prostate cancer using 66204-1-Ig

    IHC staining of human prostate cancer using 66204-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCM2 antibody included
  • IF Staining of MCF-7 using 66204-1-Ig

    IF Staining of MCF-7 using 66204-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (66204-1-Ig, Clone: 1F4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, HEK-293 cells, A431 cells, 4T1 cells, A549 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MCM2 Ready-To-Use IHC Kit. MCM2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66204-1-Ig targets MCM2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MCM2 fusion protein Ag0798 相同性解析による交差性が予測される生物種
Full Name minichromosome maintenance complex component 2
Calculated molecular weight 102 kDa
Observed molecular weight 120-125 kDa
GenBank accession numberBC007670
Gene symbol MCM2
Gene ID (NCBI) 4171
RRIDAB_2881595
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MCM2 antibody 66204-1-IgDownload protocol
IHC protocol for MCM2 antibody 66204-1-IgDownload protocol
IF protocol for MCM2 antibody 66204-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols