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Species Reactivity

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  • Featured Product
  • KD/KO Validated

MCL1 Polyclonal antibody

MCL1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16225-1-AP

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

Validation Data Gallery

Applications:
  • WB analysis using 16225-1-AP

    WB analysis using 16225-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16225-1-AP

    WB analysis using 16225-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 16225-1-AP

    WB analysis of A431 using 16225-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Raji using 16225-1-AP

    WB analysis of Raji using 16225-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 16225-1-AP

    WB analysis of HL-60 using 16225-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16225-1-AP

    WB analysis using 16225-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse spleen using 16225-1-AP

    WB analysis of mouse spleen using 16225-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human liver using 16225-1-AP

    WB analysis of human liver using 16225-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 16225-1-AP

    WB analysis of human brain using 16225-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 16225-1-AP

    WB analysis of K-562 using 16225-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16225-1-AP (MCL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 16225-1-AP

    IP experiment of Raji using 16225-1-AP

    IP result of anti-MCL1 (IP:16225-1-AP, 3ug; Detection:16225-1-AP 1:1000) with Raji cells lysate 800ug.

  • IF Staining of MCF-7 using 16225-1-AP

    IF Staining of MCF-7 using 16225-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MCL1 antibody (16225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Ramos using 16225-1-AP

    FC experiment of Ramos using 16225-1-AP

    1X10^6 Ramos cells were intracellularly stained with 0.5 ug Anti-Human MCL1 (16225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue, Raji cells, HL-60 cells, A431 cells, human liver tissue, HEK-293T cells, human brain tissue, K-562 cells, MCF-7 cells, HeLa cells
Positive IP detected inRaji cells
Positive IF detected inMCF-7 cells
Positive FC detected inRamos cells
Planning an IHC experiment? We recommend our IHCeasy MCL1 Ready-To-Use IHC Kit. MCL1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

16225-1-AP targets MCL1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCL1 fusion protein Ag10397 相同性解析による交差性が予測される生物種
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Calculated molecular weight 350 aa, 37 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC107735
Gene symbol MCL1
Gene ID (NCBI) 4170
RRIDAB_2143977
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)

Protocols

Product Specific Protocols
WB protocol for MCL1 antibody 16225-1-APDownload protocol
IF protocol for MCL1 antibody 16225-1-APDownload protocol
IP protocol for MCL1 antibody 16225-1-APDownload protocol
FC protocol for MCL1 antibody 16225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nat Commun

MCL-1 gains occur with high frequency in lung adenocarcinoma and can be targeted therapeutically.

Authors - Enkhtsetseg Munkhbaatar
View Article
humanWB

Nat Commun

Small-molecule suppression of calpastatin degradation reduces neuropathology in models of Huntington's disease.

Authors - Di Hu
View Article
mouseWB

Nat Commun

IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation.

Authors - Senthil Kumaran Satyanarayanan
View Article
humanWB

EBioMedicine

The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.

Authors - Pingbo Chen
View Article
humanWB,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li
View Article
humanWB

Aging (Albany NY)

Melatonin induces cell cycle arrest and suppresses tumor invasion in urinary bladder urothelial carcinoma

Authors - Tzuo-Yi Hsieh
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Eya (Verified Customer) (07-06-2021)

the bands appear very clear

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: shTHP1