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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MCHR1 Polyclonal antibody

MCHR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11656-2-AP

Synonyms

G protein coupled receptor 24, GPR24, MCH 1R, MCH R1, MCH receptor 1, MCH1R, MCHR, MCHR 1, MCHR1, SLC 1, SLC1

Validation Data Gallery

Applications:
  • WB analysis of SH-SY5Y using 11656-2-AP

    WB analysis of SH-SY5Y using 11656-2-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11656-2-AP (MCHR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 11656-2-AP

    IHC staining of human heart using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11656-2-AP (MCHR1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 11656-2-AP

    IHC staining of human heart using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11656-2-AP (MCHR1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human brain using 11656-2-AP

    IHC staining of human brain using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11656-2-AP (MCHR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 11656-2-AP

    IHC staining of human brain using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11656-2-AP (MCHR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 11656-2-AP

    IHC staining of mouse heart using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 11656-2-AP

    IHC staining of mouse heart using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11656-2-AP

    IHC staining of mouse brain using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11656-2-AP

    IHC staining of mouse brain using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inhuman heart tissue, human brain tissue, mouse brain tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11656-2-AP targets MCHR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MCHR1 fusion protein Ag2224 相同性解析による交差性が予測される生物種
Full Name melanin-concentrating hormone receptor 1
Calculated molecular weight 422 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC021146
Gene symbol MCHR1
Gene ID (NCBI) 2847
RRIDAB_2143940
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MCHR1 antibody 11656-2-APDownload protocol
IHC protocol for MCHR1 antibody 11656-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols