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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using MCHR1 Polyclonal antibody (11656-2-AP)

    WB analysis of SH-SY5Y using 11656-2-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11656-2-AP (MCHR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MCHR1 Polyclonal antibody (11656-2-AP)

    IHC staining of mouse heart using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using MCHR1 Polyclonal antibody (11656-2-AP)

    IHC staining of human brain using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11656-2-AP (MCHR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MCHR1 Polyclonal antibody (11656-2-AP)

    IHC staining of mouse heart using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MCHR1 Polyclonal antibody (11656-2-AP)

    IHC staining of mouse heart using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MCHR1 Polyclonal antibody (11656-2-AP)

    IHC staining of mouse brain using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MCHR1 Polyclonal antibody (11656-2-AP)

    IHC staining of mouse brain using 11656-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11656-2-AP (MCHR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MCHR1 Polyclonal antibody

MCHR1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11656-2-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MCH receptor 1, MCH R1, MCH 1R, G-protein coupled receptor 24, GPR24

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse heart tissue, human brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11656-2-AP targets MCHR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2224

Product name: Recombinant human MCHR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-422 aa of BC021146

Sequence: MSVGAMKKGVGRAVGLGGGSGCQATEEDPLPDCGACAPGQGGRRWRLPQPAWVEGSSARLWEQATGTGWMDLEASLLPTGPNASNTSDGPDNLTSAGSPPRTGSISYINIIMPSVFGTICLLGIIGNSTVIFAVVKKSKLHWCNNVPDIFIINLSVVDLLFLLGMPFMIHQLMGNGVWHFGETMCTLITAMDANSQFTSTYILTAMAIDRYLATVHPISSTKFRKPSVATLVICLLWALSFISITPVWLYARLIPFPGGAVGCGIRLPNPDTDLYWFTLYQFFLAFALPFVVITAAYVRILQRMTSSVAPASQRSIRLRTKRVTRTAIAICLVFFVCWAPYYVLQLTQLSISRPTLTFVYLYNAAISLGYANSCLNPFVYIVLCETFRKRLVLSVKPAAQGQLRAVSNAQTADEERTESKGT

相同性解析による交差性が予測される生物種
Full Name melanin-concentrating hormone receptor 1
Calculated molecular weight 422 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC021146
Gene Symbol MCHR1
Gene ID (NCBI) 2847
RRIDAB_2143940
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99705
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for MCHR1 antibody 11656-2-APDownload protocol
WB protocol for MCHR1 antibody 11656-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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