Myoglobin Polyclonal antibody

Myoglobin Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16048-1-AP

Synonyms

MB, MYO, myoglobin



Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle, rat heart, rat skeletal muscle
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

16048-1-AP targets Myoglobin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Myoglobin fusion protein Ag9000 相同性解析による交差性が予測される生物種
Full Name myoglobin
Calculated molecular weight 154 aa, 17 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC014547
Gene symbol MB
Gene ID (NCBI) 4151
RRIDAB_1640050
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.

Protocols

Product Specific Protocols
WB protocol for Myoglobin antibody 16048-1-APDownload protocol
IHC protocol for Myoglobin antibody 16048-1-APDownload protocol
IP protocol for Myoglobin antibody 16048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Physiol Rep

Effects of endurance training on the expression of host proteins involved in SARS-CoV-2 cell entry in C57BL/6J mouse.

Authors - Yuki Tamura
mouseWB

Appl Physiol Nutr Metab

Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle

Authors - Song Huang