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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated MAdCAM1 Monoclonal antibody

MAdCAM1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A12E8

Cat no : CL594-66594

Synonyms

hMAdCAM 1, MACAM1, MAdCAM 1, MADCAM1

Validation Data Gallery

Applications:
  • IF Staining of human tonsillitis using CL594-66594

    IF Staining of human tonsillitis using CL594-66594

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 MAdCAM1 antibody (CL594-66594, Clone: 2A12E8 ) at dilution of 1:200.

Tested Applications

Positive IF detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 66594-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66594 targets MAdCAM1 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MAdCAM1 fusion protein Ag16184 相同性解析による交差性が予測される生物種
Full Name mucosal vascular addressin cell adhesion molecule 1
Calculated molecular weight 406 aa, 43 kDa
GenBank accession numberBC142629
Gene symbol MADCAM1
Gene ID (NCBI) 8174
RRIDAB_2920014
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.

Protocols

Product Specific Protocols
IF protocol for CL594 MAdCAM1 antibody CL594-66594Download protocol
Standard Protocols
Click here to view our Standard Protocols