Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, SGC-7901cells, NIH/3T3 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach cancer tissue, human colon cancer tissue, human kidney tissue, mouse liver tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 18 publications below |
| IHC | See 3 publications below |
| IF | See 4 publications below |
Product Information
55309-1-AP targets MAT2A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | methionine adenosyltransferase II, alpha |
| Calculated molecular weight | 44 kDa |
| Observed molecular weight | 47 kDa, 49 kDa |
| GenBank accession number | NM_005911 |
| Gene Symbol | MAT2A |
| Gene ID (NCBI) | 4144 |
| RRID | AB_2881303 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31153 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MAT2A, also named as AMS2, MATA2 and AdoMet synthase 2, belongs to the AdoMet synthase family. It catalyzes the formation of S-adenosylmethionine from methionine and ATP. This antibody is specific to MATA2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAT2A antibody 55309-1-AP | Download protocol |
| IHC protocol for MAT2A antibody 55309-1-AP | Download protocol |
| IP protocol for MAT2A antibody 55309-1-AP | Download protocol |
| WB protocol for MAT2A antibody 55309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.
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Oxid Med Cell Longev Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through m6A Modification of MAT2A Pre-mRNA by METTL16.
| ||
Antioxidants (Basel) M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide | ||
FASEB J Activating transcription factor 4 regulates angiogenesis under lipid overload via methionine adenosyltransferase 2A-mediated endothelial epigenetic alteration.
| ||
FASEB J Inhibition of MAT2A suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss.
| ||
Environ Toxicol Pharmacol Ligustrazine modulates renal cysteine biosynthesis in rats exposed to cadmium. |
