TAU Polyclonal antibody

TAU Polyclonal Antibody for WB, IHC, IF-Fro, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-Fro, ELISA

Conjugate

Unconjugated

Cat no : 10274-1-AP

Synonyms

MAPT, Paired helical filament tau, Microtubule-associated protein tau, MAPTL, DDPAC


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Tested Applications

Positive WB detected inmouse brain tissue, rat brain
Positive IHC detected inhuman brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10274-1-AP targets TAU in WB, IHC, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAU fusion protein Ag0354 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein tau
Calculated molecular weight 37-46, 79-81 kDa
Observed molecular weight 45-70 kDa, 100 kDa
GenBank accession numberBC000558
Gene symbol TAU
Gene ID (NCBI) 4137
RRIDAB_2139718
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Present polyclonal anti-Tau antibody was produced by immunizing animals with N-terminal of Tau and can detect approx 45-70 kDa and 100-kDa bands in brain tissues.

Protocols

Product Specific Protocols
WB protocol for TAU antibody 10274-1-APDownload protocol
IHC protocol for TAU antibody 10274-1-APDownload protocol
IF protocol for TAU antibody 10274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.

Authors - Xinwen Zhang
human

Brain

Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.

Authors - Lina Pan
mouseIHC

Hum Mol Genet

Parkin deletion causes cerebral and systemic amyloidosis in human mutated tau over-expressing mice.

Authors - Rodríguez-Navarro Jose A JA
ratWB

Acta Neuropathol Commun

Laterality and region-specific tau phosphorylation correlate with PTSD-related behavioral traits in rats exposed to repetitive low-level blast.

Authors - Georgina Perez Garcia
mouse,ratWB

Free Radic Biol Med

The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro

Authors - Jie Ai
mouseIF

Mol Ther Nucleic Acids

Implications of miR-148a-3p/p35/PTEN signaling in tau hyperphosphorylation and autoregulatory feedforward of Akt/CREB in Alzheimer's disease.

Authors - Li Zeng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

manohar (Verified Customer) (03-06-2024)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Neuronal stem cells
FH

David (Verified Customer) (01-02-2024)

Produces a single band at around 45kDa which is concordant with the data sheet.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: SH-SY5Y lysate
FH

Tanusree (Verified Customer) (02-14-2019)

The antibody worked well with the mouse primary cells and tissue.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse brain