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EB1 Polyclonal antibody

EB1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17717-1-AP

Synonyms

APC binding protein EB1, EB1, End binding protein 1, MAPRE1



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, A431 cells
Positive IP detected inHeLa cells, Caco-2 cells, mouse thymus tissue
Positive IHC detected inhuman kidney tissue, human colon cancer tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 4 publications below

Product Information

17717-1-AP targets EB1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EB1 fusion protein Ag12092 相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein, RP/EB family, member 1
Calculated molecular weight 268 aa, 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC106068
Gene symbol MAPRE1
Gene ID (NCBI) 22919
RRIDAB_2141624
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EB1 antibody 17717-1-APDownload protocol
IHC protocol for EB1 antibody 17717-1-APDownload protocol
IP protocol for EB1 antibody 17717-1-APDownload protocol
FC protocol for EB1 antibody 17717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nat Commun

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Authors - Hai-Qing Tu
humanIF

Elife

α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.

Authors - Dandan Ma
mouseIF

Biol Open

MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1

Authors - Peihong Su
mouseIF

Biol Open

MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1.

Authors - Peihong Su
humanIF

Molecules

Pyrrolyldihydropyrazino[1,2-a]indoletrione Analogue Microtubule Inhibitor Induces Cell-Cycle Arrest and Apoptosis in Colorectal Cancer Cells

Authors - Dong-Lin Yang