Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells, mouse liver tissue, human liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 15 publications below |
| IHC | See 8 publications below |
| IF | See 8 publications below |
Product Information
12602-1-AP targets MAOB in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MAOB fusion protein Ag3253 相同性解析による交差性が予測される生物種 |
| Full Name | monoamine oxidase B |
| Calculated molecular weight | 520 aa, 59 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC022494 |
| Gene Symbol | MAOB |
| Gene ID (NCBI) | 4129 |
| RRID | AB_2137273 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27338 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position (PMID:3387449).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAOB antibody 12602-1-AP | Download protocol |
| IHC protocol for MAOB antibody 12602-1-AP | Download protocol |
| IP protocol for MAOB antibody 12602-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Social deprivation induces astrocytic TRPA1-GABA suppression of hippocampal circuits | ||
EMBO Rep Regional heterogeneity of astrocyte morphogenesis dictated by the formin protein, Daam2, modifies circuit function. | ||
J Transl Med Identification and experimental validation of KMO as a critical immune-associated mitochondrial gene in unstable atherosclerotic plaque | ||
Br J Pharmacol Andrographolide alleviates parkinsonism in MPTP-PD mice via targeting DRP1-mediated mitochondrial fission. | ||
Aging (Albany NY) A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients. | ||
Aging (Albany NY) Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis. |