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  • KD/KO Validated

MAGT1 Polyclonal antibody

MAGT1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17430-1-AP

Synonyms

IAG2, IAP, magnesium transporter 1, MAGT1, OST3B, PRO0756, UNQ628/PRO1244



Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse heart tissue, mouse colon tissue, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman liver tissue, human colon cancer tissue, human skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17430-1-AP targets MAGT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGT1 fusion protein Ag11496 相同性解析による交差性が予測される生物種
Full Name magnesium transporter 1
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 45-47 kDa
GenBank accession numberBC060842
Gene symbol MAGT1
Gene ID (NCBI) 84061
RRIDAB_2138982
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAGT1 is a mammalian Mg2+-selective transporter being required for cellular magnesium uptake and vertebrate embryonic development. It possesses five putative transmembrane (TM) regions with a cleavage site, a N-glycosylation site, and a number of phosphorylation sites. Recently mutations in MAGT1 has been found to be asscociated with a novel X-linked human immunodeficiency. The MAGT1 protein was undetectable in the patients'cells by western blot or immunofluorescent cell surface staining. MAGT1 had been detected as 38 kDa (PMID: 18705540) or 45-47 kDa (PMID: 27383987) by western blot.

Protocols

Product Specific Protocols
WB protocol for MAGT1 antibody 17430-1-APDownload protocol
IHC protocol for MAGT1 antibody 17430-1-APDownload protocol
IF protocol for MAGT1 antibody 17430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nature

Second messenger role for Mg2+ revealed by human T-cell immunodeficiency.

Authors - Li Feng-Yen FY
humanWB

Nature

Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens.

Authors - Caleb D Marceau
  • KO Validated
ratWB,IF

Adv Sci (Weinh)

A Magnesium-Enriched 3D Culture System that Mimics the Bone Development Microenvironment for Vascularized Bone Regeneration.

Authors - Sihan Lin
ratWB,IF

Small

Magnetic Field Boosts the Transmembrane Transport Efficiency of Magnesium Ions from PLLA Bone Scaffold

Authors - Zuyun Yan
humanWB, IF

J Cell Biol

Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.

Authors - Natalia A Cherepanova
  • KD Validated
humanWB

MBio

Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.

Authors - David L Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Natalia (Verified Customer) (09-26-2019)

To my knowledge, this one is the only one commercially available antibody that works well for WB application to detect MagT1. It cross-reacts with TUSC3 a little, only because these are highly homological proteins.

  • Applications: Western Blot, Immunofluorescence,