Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of human brain using 12683-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12683-1-AP
WB result of MAD2L2 antibody (12683-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAD2L2 transfected HEK-293 cells.
WB analysis of HeLa using 12683-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human colon using 12683-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 12683-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12683-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12683-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12683-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 12683-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12683-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12683-1-AP
IP result of anti-MAD2L2 (IP:12683-1-AP, 3ug; Detection:12683-1-AP 1:500) with mouse brain tissue lysate 3600ug.
IHC staining of human ovary tumor using 12683-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 12683-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 12683-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 12683-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

MAD2L2 Polyclonal antibody

MAD2L2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12683-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

hREV7, MAD2 like protein 2, MAD2B, MAD2L2, REV7, REV7 homolog

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (21)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human brain tissue, A375 cells, human kidney tissue, human colon tissue, mouse brain tissue, HeLa cells, HEK-293 cells, K-562 cells, fetal human brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human ovary tumor tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 20 publications below
IHC	See 2 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

12683-1-AP targets MAD2L2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3373 Product name: Recombinant human MAD2L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-211 aa of BC015244 Sequence: TTLTRQDLNFGQVVADVLCEFLEVAVHLILYVREVYPVGIFQKRKKYNVPVQMSCHPELNQYIQDTLHCVKPLLEKNDVEKVVVVILDKEHRPVEKFVFEITQPPLLSISSDSLLSHVEQLLRAFILKISVCDAVLDHNPPGCTFTVLVHTREAATRNMEKIQVIKDFPWILADEQDVHMHDPRLIPLKTMTSDILKMQLYVEERAHKGS 相同性解析による交差性が予測される生物種
Full Name	MAD2 mitotic arrest deficient-like 2 (yeast)
Calculated molecular weight	211 aa, 24 kDa
Observed molecular weight	24 kDa
GenBank accession number	BC015244
Gene Symbol	MAD2L2
Gene ID (NCBI)	10459
RRID	AB_2139530
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UI95
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.

Protocols

Product Specific Protocols
IHC protocol for MAD2L2 antibody 12683-1-AP	Download protocol
IP protocol for MAD2L2 antibody 12683-1-AP	Download protocol
WB protocol for MAD2L2 antibody 12683-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair. Authors - Inge de Krijger KO Validated View Article
human	WB	Nat Commun The RING finger E3 ligase RNF25 protects DNA replication forks independently of its canonical roles in ubiquitin signaling Authors - Lilly F Chiou View Article
mouse	WB	Nat Commun SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination. Authors - Estelle Vincendeau View Article
human	WB,IP	Nucleic Acids Res REV7 associates with ATRIP and inhibits ATR kinase activity. Authors - Megan Biller KO Validated View Article
human	WB	Nucleic Acids Res REV1 and polymerase ζ facilitate homologous recombination repair. Authors - Sharma Shilpy S KD Validated View Article
human	WB	Nucleic Acids Res Regulation of error-prone translesion synthesis by Spartan/C1orf124. Authors - Kim Myoung Shin MS View Article

Validation Data Gallery

WB analysis of human brain using 12683-1-AP

WB analysis of HEK-293 using 12683-1-AP

WB analysis of HeLa using 12683-1-AP

WB analysis of human colon using 12683-1-AP

WB analysis of A375 using 12683-1-AP

WB analysis of mouse brain using 12683-1-AP

WB analysis of HEK-293 using 12683-1-AP

WB analysis of K-562 using 12683-1-AP

WB analysis of fetal human brain using 12683-1-AP

WB analysis of human kidney using 12683-1-AP

IP experiment of mouse brain using 12683-1-AP

IHC staining of human ovary tumor using 12683-1-AP

IHC staining of human ovary tumor using 12683-1-AP

IHC staining of human lymphoma using 12683-1-AP

IHC staining of human lymphoma using 12683-1-AP

MAD2L2 Polyclonal antibody

MAD2L2 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair.

The RING finger E3 ligase RNF25 protects DNA replication forks independently of its canonical roles in ubiquitin signaling

SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination.

REV7 associates with ATRIP and inhibits ATR kinase activity.

REV1 and polymerase ζ facilitate homologous recombination repair.

Regulation of error-prone translesion synthesis by Spartan/C1orf124.

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