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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Mouse Ly-6G (1A8)

Ly-6G Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

1A8

Cat no : PE-65078

Synonyms

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G

Validation Data Gallery

Applications:
  • FC experiment of BALB/c mouse bone marrow cells using PE-65078

    FC experiment of BALB/c mouse bone marrow cells using PE-65078

    1X10^6 BALB/c mouse bone marrow cells were surface stained with 0.5 ug PE Anti-Mouse Ly-6G (PE-65078, Clone: 1A8) or 0.5 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3). Cells were not fixed.

  • FC experiment of BALB/c mouse bone marrow cells using PE-65078

    FC experiment of BALB/c mouse bone marrow cells using PE-65078

    1X10^6 BALB/c mouse bone marrow cells were surface stained with 0.5 ug PE Anti-Mouse Ly-6G (PE-65078, Clone: 1A8) (red), or 0.5 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3) (blue). Cells were not fixed.

Tested Applications

Positive FC detected inmouse bone marrow cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

PE-65078 targets Ly-6G in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Ly-6G transfected EL-4J cell line 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 6 complex, locus G
GenBank accession numberX70920
Gene symbol Ly6g
Gene ID (NCBI) 546644
RRIDAB_2883874
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).

Protocols

Product Specific Protocols
FC protocol for PE Ly-6G antibody PE-65078Download protocol
Standard Protocols
Click here to view our Standard Protocols