CoraLite® Plus 488 Anti-Mouse Ly-6G (1A8)

Ly-6G Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A8

Cat no : CL488-65078

Synonyms

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G



Tested Applications

Positive FC detected inmouse bone marrow cells
For other applications, we recommend the unconjugated version of this antibody, 65078-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65078 targets Ly-6G in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Ly-6G transfected EL-4J cell line 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 6 complex, locus G
GenBank accession numberX70920
Gene symbol Ly6g
Gene ID (NCBI) 546644
RRIDAB_2883164
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Ly-6G antibody CL488-65078Download protocol
Standard Protocols
Click here to view our Standard Protocols