Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse stomach tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
18216-1-AP targets LSR in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12961 Product name: Recombinant human LSR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 339-589 aa of BC004381 Sequence: SMRVLYYMEKELANFDPSRPGPPSGRVERAMSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRRSRAYMPPRSRSRDDLYDQDDSRDFPRSRDPHYDDFRSRERPPADPRSHHHRTRDPRDNGSRSGDLPYDGRLLEEAVRKKGSEERRRPHKEEEEEAYYPPAPPPYSETDSQASRERRLKKNLALSRESLVV 相同性解析による交差性が予測される生物種 |
| Full Name | lipolysis stimulated lipoprotein receptor |
| Calculated molecular weight | 71 kDa |
| Observed molecular weight | 60-70 kDa, 56 kDa |
| GenBank accession number | BC004381 |
| Gene Symbol | LSR |
| Gene ID (NCBI) | 51599 |
| RRID | AB_2250137 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86X29 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LSR (lipolysis stimulated lipoprotein receptor) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids (PMID: 10224102). This remnant receptor binds both apoB- and apoE-containing lipoproteins and displays greatest affinity for TG-rich lipoproteins. Distribution of LSR causes embryonic lethality in mice, indicating that the expression of LSR is critical for liver and embryonic development (PMID: 15265030). Investigations show that LSR is a multimer consisting of α subunit (68 kDa) and β subunit (56 kDa) associated through disulfide bridges (PMID: 10224102; 19188430; 18644789).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LSR antibody 18216-1-AP | Download protocol |
| WB protocol for LSR antibody 18216-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biosens Bioelectron A multiple mixed TiO2 mesocrystal junction based PEC-colorimetric immunoassay for specific recognition of lipolysis stimulated lipoprotein receptor. | ||
J Hazard Mater Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice. |
