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  • Featured Product
  • KD/KO Validated

LRPPRC Monoclonal antibody

LRPPRC Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3H7E1

Cat no : 67679-1-Ig

Synonyms

130 kDa leucine rich protein, CLONE 23970, GP130, LRP 130, LRP130, LRPPRC, LSFC

Validation Data Gallery

Applications:
  • WB analysis using 67679-1-Ig

    WB analysis using 67679-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67679-1-Ig

    WB analysis of HeLa using 67679-1-Ig

    WB result of LRPPRC antibody (67679-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LRPPRC transfected HeLa cells.

  • WB analysis of U-251 using 67679-1-Ig

    WB analysis of U-251 using 67679-1-Ig

    U-251 cells were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67679-1-Ig

    IHC staining of human colon cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human colon cancer using 67679-1-Ig

    IHC staining of human colon cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human endometrial cancer using 67679-1-Ig

    IHC staining of human endometrial cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human endometrial cancer using 67679-1-Ig

    IHC staining of human endometrial cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human liver cancer using 67679-1-Ig

    IHC staining of human liver cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human liver cancer using 67679-1-Ig

    IHC staining of human liver cancer using 67679-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IF Staining of HeLa using 67679-1-Ig

    IF Staining of HeLa using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HEK-293 using 67679-1-Ig

    IF Staining of HEK-293 using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin is stained using CoraLite®555-phalloidin (red) and DNA is stained by DAPI (blue).

  • IF Staining of HepG2 using 67679-1-Ig

    IF Staining of HepG2 using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of A431 using 67679-1-Ig

    IF Staining of A431 using 67679-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inU2OS cells, U-251 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human endometrial cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HEK-293 cells, HepG2 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy LRPPRC Ready-To-Use IHC Kit. LRPPRC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67679-1-Ig targets LRPPRC in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LRPPRC fusion protein Ag30261 相同性解析による交差性が予測される生物種
Full Name leucine-rich PPR-motif containing
Calculated molecular weight 1394 aa, 158 kDa
Observed molecular weight130 kDa
GenBank accession numberBC050311
Gene symbol LRPPRC
Gene ID (NCBI) 10128
RRIDAB_2882872
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

Protocols

Product Specific Protocols
WB protocol for LRPPRC antibody 67679-1-IgDownload protocol
IHC protocol for LRPPRC antibody 67679-1-IgDownload protocol
IF protocol for LRPPRC antibody 67679-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Genet

RNA methylation pattern and immune microenvironment characteristics mediated by m6A regulator in ischemic stroke

Authors - Kejuan Jia
View Article