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Species Reactivity

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  • Featured Product
  • KD/KO Validated

LPCAT1 Polyclonal antibody

LPCAT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16112-1-AP

Synonyms

Acyltransferase like 2, AYTL2, LPC acyltransferase 1, lpcat, LPCAT 1, LPCAT1, Lyso PAF acetyltransferase, LysoPAFAT, LysoPC acyltransferase 1, PFAAP3

Validation Data Gallery

Applications:
  • WB analysis using 16112-1-AP

    WB analysis using 16112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16112-1-AP

    WB analysis using 16112-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 16112-1-AP

    WB analysis of rat brain using 16112-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 16112-1-AP

    WB analysis of mouse brain using 16112-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of human lung using 16112-1-AP

    WB analysis of human lung using 16112-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse spleen using 16112-1-AP

    WB analysis of mouse spleen using 16112-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16112-1-AP (LPCAT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 16112-1-AP

    IP experiment of mouse brain using 16112-1-AP

    IP result of anti-LPCAT1 (IP:16112-1-AP, 3ug; Detection:16112-1-AP 1:4000) with mouse brain tissue lysate 3600ug.

  • IP experiment of mouse brain using 16112-1-AP

    IP experiment of mouse brain using 16112-1-AP

    IP result of anti-LPCAT1 (IP:16112-1-AP, 4ug; Detection:16112-1-AP 1:4000) with mouse brain tissue lysate 1280 ug.

  • IHC staining of human breast cancer using 16112-1-AP

    IHC staining of human breast cancer using 16112-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 16112-1-AP

    IHC staining of human breast cancer using 16112-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16112-1-AP

    IHC staining of human colon cancer using 16112-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 16112-1-AP

    IHC staining of mouse lung using 16112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 16112-1-AP

    IHC staining of mouse spleen using 16112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 16112-1-AP

    IHC staining of mouse spleen using 16112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 16112-1-AP

    IHC staining of mouse lung using 16112-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16112-1-AP

    IHC staining of human colon cancer using 16112-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16112-1-AP (LPCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 16112-1-AP

    IF Staining of MCF-7 using 16112-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 16112-1-AP (LPCAT1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, human lung tissue, mouse spleen tissue, mouse brain tissue, rat brain tissue, A549 cells, mouse lung tissue, rat lung tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, mouse spleen tissue, mouse lung tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16112-1-AP targets LPCAT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPCAT1 fusion protein Ag9035 相同性解析による交差性が予測される生物種
Full Name lysophosphatidylcholine acyltransferase 1
Calculated molecular weight 534 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC020166
Gene symbol LPCAT1
Gene ID (NCBI) 79888
RRIDAB_2135554
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).

Protocols

Product Specific Protocols
WB protocol for LPCAT1 antibody 16112-1-APDownload protocol
IHC protocol for LPCAT1 antibody 16112-1-APDownload protocol
IF protocol for LPCAT1 antibody 16112-1-APDownload protocol
IP protocol for LPCAT1 antibody 16112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Metab

Oncogene Amplification in Growth Factor Signaling Pathways Renders Cancers Dependent on Membrane Lipid Remodeling.

Authors - Junfeng Bi
  • KD Validated
View Article
humanWB

J Hepatol

Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression.

Authors - Morita Yoshifumi Y
  • KD Validated
View Article
HUMANWB

Curr Biol

Neutral lipid stores and lipase PNPLA5 contribute to autophagosome biogenesis.

Authors - Nicolas Dupont
View Article
humanWB,IF

Proc Natl Acad Sci U S A

Regulation of stem cell function and neuronal differentiation by HERV-K via mTOR pathway.

Authors - Tongguang Wang
  • KD Validated
View Article
humanIHC

Elife

Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.

Authors - Marko Z Nikolić
View Article
humanWB

Front Immunol

Identification of molecular subtypes based on PANoptosis-related genes and construction of a signature for predicting the prognosis and response to immunotherapy response in hepatocellular carcinoma

Authors - Jinfeng Zhu
  • KD Validated
View Article