Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, human placenta tissue, A375 cells, human heart tissue |
| Positive IHC detected in | rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
67139-1-Ig targets LOXL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.
| Tested Reactivity | Human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28566 Product name: Recombinant human LOXL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 129-293 aa of BC000594 Sequence: TGNEATLAACTSNGWGVTDCKHTEDVGVVCSDKRIPGFKFDNSLINQIENLNIQVEDIRIRAILSTYRKRTPVMEGYVEVKEGKTWKQICDKHWTAKNSRVVCGMFGFPGERTYNTKVYKMFASRRKQRYWPFSMDCTGTEAHISSCKLGPQVSLDPMKNVTCEN 相同性解析による交差性が予測される生物種 |
| Full Name | lysyl oxidase-like 2 |
| Calculated molecular weight | 774 aa, 87 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC000594 |
| Gene Symbol | LOXL2 |
| Gene ID (NCBI) | 4017 |
| RRID | AB_2882438 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y4K0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LOXL2, also named as WS9-14, belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. LOXL2 activity might be a crucial modulator of Snail, providing an additional control mechanism of EMT and tumor progression. Increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression. We detected a band about 100 kd which is a secretory mature LOXL2 protein modified by glycosylation (PMID: 24014025).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LOXL2 antibody 67139-1-Ig | Download protocol |
| IHC protocol for LOXL2 antibody 67139-1-Ig | Download protocol |
| WB protocol for LOXL2 antibody 67139-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest LOXL2-induced PEAR1 Ser891 phosphorylation suppresses CD44 degradation and promotes triple-negative breast cancer metastasis | ||
Aging Cell Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis. | ||
Int J Biol Macromol Depletion of LOXL2 improves respiratory capacity: From air-breathing fish to mammal under hypoxia. | ||
Oncogene METTL1/WDR4-mediated tRNA m7G modification and mRNA translation control promote oncogenesis and doxorubicin resistance | ||
Neuron Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier | ||
Neoplasia β-Catenin mediated TAM phenotype promotes pancreatic cancer metastasis via the OSM/STAT3/LOXL2 axis |
