Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, COLO 320 cells, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 5 publications below |
| ELISA | See 1 publications below |
Product Information
16602-1-AP targets LIPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9775 Product name: Recombinant human LIPH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 103-451 aa of BC064941 Sequence: VVVVDWNRGATTLIYTHASSKTRKVAMVLKEFIDQMLAEGASLDDIYMIGVSLGAHISGFVGEMYDGWLGRITGLDPAGPLFNGKPHQDRLDPSDAQFVDVIHSDTDALGYKEPLGNIDFYPNGGLDQPGCPKTILGGFQYFKCDHQRSVYLYLSSLRESCTITAYPCDSYQDYRNGKCVSCGTSQKESCPLLGYYADNWKDHLRGKDPPMTKAFFDTAEESPFCMYHYFVDIITWNKNVRRGDITIKLRDKAGNTTESKINHEPTTFQKYHQVSLLARFNQDLDKVAAISLMFSTGSLIGPRYKLRILRMKLRSLAHPERPQLCRYDLVLMENVETVFQPILCPELQL 相同性解析による交差性が予測される生物種 |
| Full Name | lipase, member H |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC064941 |
| Gene Symbol | LIPH |
| Gene ID (NCBI) | 200879 |
| RRID | AB_2135352 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWY8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LIPH antibody 16602-1-AP | Download protocol |
| IHC protocol for LIPH antibody 16602-1-AP | Download protocol |
| IP protocol for LIPH antibody 16602-1-AP | Download protocol |
| WB protocol for LIPH antibody 16602-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genet Med Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis. | ||
Cell Oncol (Dordr) Genomic analysis and filtration of novel prognostic biomarkers based on metabolic and immune subtypes in pancreatic cancer | ||
J Cell Mol Med LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer.
| ||
Tumour Biol Lipase member H is a novel secreted protein associated with a poor prognosis for breast cancer patients. | ||
Biochem Biophys Res Commun Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas. | ||
Tumour Biol Lipase member H frequently overexpressed in human esophageal adenocarcinomas. |
