Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 20772-1-AP
WB result of LIMS1 antibody (20772-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMS1 transfected HeLa cells.
WB analysis using 20772-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 20772-1-AP
Caco-2 cells were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 20772-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 20772-1-AP
IP result of anti-LIMS1 (IP:20772-1-AP, 4ug; Detection:20772-1-AP 1:800) with K-562 cells lysate 1640 ug.
IHC staining of human colon cancer using 20772-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20772-1-AP (LIMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 20772-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20772-1-AP (LIMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 20772-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LIMS1 antibody (20772-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HeLa using 20772-1-AP
Immunofluorescent analysis of Hela cells, using LIMS1 antibody 20772-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

LIMS1 Polyclonal antibody

LIMS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20772-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH, PINCH 1, PINCH1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Caco-2 cells, human kidney tissue, HeLa cells, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IP	See 1 publications below

Product Information

20772-1-AP targets LIMS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14764 Product name: Recombinant human LIMS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC005341 Sequence: MANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFTIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGGVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLAETLGRK 相同性解析による交差性が予測される生物種
Full Name	LIM and senescent cell antigen-like domains 1
Calculated molecular weight	325 aa, 37 kDa
Observed molecular weight	35-37 kDa
GenBank accession number	BC005341
Gene Symbol	LIMS1
Gene ID (NCBI)	3987
RRID	AB_10695765
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48059
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LIMS1, also known as PINCH1, is an adaptor protein that contains five LIM domains, or double zinc fingers(PMID: 7517666). PINCH-1 is a widely expressed focal adhesion protein that forms a ternary complex with integrin-linked kinase (ILK) and CH-ILKBP/actopaxin/alpha-parvin (PMID: 14551191). LIMS1 is involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques.

Protocols

Product Specific Protocols
IF protocol for LIMS1 antibody 20772-1-AP	Download protocol
IHC protocol for LIMS1 antibody 20772-1-AP	Download protocol
IP protocol for LIMS1 antibody 20772-1-AP	Download protocol
WB protocol for LIMS1 antibody 20772-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth. Authors - Ling Guo KO Validated View Article
human	WB,IP	J Cell Biol A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation. Authors - Ling Guo KD Validated View Article
human	WB	Theranostics PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex. Authors - Xiaoxiao Wang View Article
	WB	J Cell Sci Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation. Authors - Clotilde Huet-Calderwood View Article
human	WB	Amino Acids PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma. Authors - Chunhong Cui KD Validated View Article
human	WB	Cancer Med The RNA-binding protein RBMS3 inhibits the progression of colon cancer by regulating the stability of LIMS1 mRNA Authors - Yafei Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 20772-1-AP

WB analysis using 20772-1-AP

WB analysis of Caco-2 using 20772-1-AP

WB analysis of human kidney using 20772-1-AP

IP experiment of K-562 using 20772-1-AP

IHC staining of human colon cancer using 20772-1-AP

IHC staining of human colon cancer using 20772-1-AP

IF Staining of HeLa using 20772-1-AP

IF Staining of HeLa using 20772-1-AP

LIMS1 Polyclonal antibody

LIMS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.

A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.

PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.

Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.

PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

The RNA-binding protein RBMS3 inhibits the progression of colon cancer by regulating the stability of LIMS1 mRNA