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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using LILRB4 Monoclonal antibody (67119-1-Ig)

    WB analysis of MCF-7 using 67119-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using LILRB4 Monoclonal antibody (67119-1-Ig)

    WB analysis of A549 using 67119-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using LILRB4 Monoclonal antibody (67119-1-Ig)

    WB analysis of HUVEC using 67119-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using LILRB4 Monoclonal antibody (67119-1-Ig)

    IF Staining of A549 using 67119-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LILRB4 antibody (67119-1-Ig, Clone: 1C8E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

ILT-3/LILRB4 Monoclonal antibody

ILT-3/LILRB4 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67119-1-Ig
Clone No.1C8E1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

LILRB4, Immunoglobulin-like transcript 3, ILT-3, ILT3, CD85k

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, HUVEC cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67119-1-Ig targets ILT-3/LILRB4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3219

Product name: Recombinant human LILRB4 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 18-262 aa of BC026309

Sequence: RTDMQAGPLPKPTLWAEPGSVISWGNSVTIWCQGTLEAREYRLDKEESPAPWDRQNPLEPKNKARFSIPSMTEDYAGRYRCYYRSPVGWSQPSDPLELVMTGAYSKPTLSALPSPLVTSGKSVTLLCQSRSPMDTFLLIKERAAHPLLHLRSEHGAQQHQAEFPMSPVTSVHGGTYRCFSSHGFSHYLLSHPSDPLELIVSGSLEGPRPSPTRSVSTAAGPEDQPLMPTGSVPHSGLRRHWEVLI

相同性解析による交差性が予測される生物種
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
Calculated molecular weight 448 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC026309
Gene Symbol LILRB4
Gene ID (NCBI) 11006
RRIDAB_2882422
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8NHJ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for ILT-3/LILRB4 antibody 67119-1-IgDownload protocol
WB protocol for ILT-3/LILRB4 antibody 67119-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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