Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human spleen tissue, human brain tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 15 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
18051-1-AP targets DNA Ligase I in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12508 Product name: Recombinant human LIG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 670-919 aa of BC108318 Sequence: LVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY 相同性解析による交差性が予測される生物種 |
| Full Name | ligase I, DNA, ATP-dependent |
| Calculated molecular weight | 919 aa, 102 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC108318 |
| Gene Symbol | LIG1 |
| Gene ID (NCBI) | 3978 |
| RRID | AB_2265726 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18858 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 18051-1-AP is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DNA Ligase I antibody 18051-1-AP | Download protocol |
| IP protocol for DNA Ligase I antibody 18051-1-AP | Download protocol |
| WB protocol for DNA Ligase I antibody 18051-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma | ||
Nat Commun Cas9-induced large deletions and small indels are controlled in a convergent fashion. | ||
J Clin Invest Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.
| ||
Proc Natl Acad Sci U S A Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination.
| ||
Proc Natl Acad Sci U S A Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.
|
