Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse lung tissue, rat lung tissue | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below | 
| IHC | See 2 publications below | 
| IF | See 3 publications below | 
Product Information
11183-1-AP targets LHX4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat | 
| Cited Reactivity | mouse, monkey, chicken | 
| Host / Isotype | Rabbit / IgG | 
| Class | Polyclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag1642 Product name: Recombinant human LHX4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-390 aa of BC011759 Sequence: MMQSATVPAEGAVKGLPEMLGVPMQQIPQCAGCNQHILDKFILKVLDRHWHSSCLKCADCQMQLADRCFSRAGSVYCKEDFFKRFGTKCTACQQGIPPTQVVRKAQDFVYHLHCFACIICNRQLATGDEFYLMEDGRLVCKEDYETAKQNDDSEAGAKRPRTTITAKQLETLKNAYKNSPKPARHVREQLSSETGLDMRVVQVWFQNRRAKEKRLKKDAGRHRWGQFYKSVKRSRGSSKQEKESSAEDCGVSDSELSFREDQILSELGHTNRIYGNVGDVTGGQLMNGSFSMDGTGQSYQDLRDGSPYGIPQSPSSISSLPSHAPLLNGLDYTVDSNLGIIAHAGQGVSQTLRAMAGGPTSDISTGSSVGYPDFPTSPGSWLDEMDHPPF相同性解析による交差性が予測される生物種 | 
| Full Name | LIM homeobox 4 | 
| Calculated molecular weight | 43 kDa | 
| Observed molecular weight | 43-47 kDa | 
| GenBank accession number | BC011759 | 
| Gene Symbol | LHX4 | 
| Gene ID (NCBI) | 89884 | 
| RRID | AB_2858194 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Antigen affinity purification | 
| UNIPROT ID | Q969G2 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LHX4 antibody 11183-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Sci Rep Notch signaling represses cone photoreceptor formation through the regulation of retinal progenitor cell states. | ||
| Invest Ophthalmol Vis Sci Identification of Genes With Enriched Expression in Early Developing Mouse Cone Photoreceptors. | ||
| Cell Rep LIM-Homeodomain Transcription Factor LHX4 Is Required for the Differentiation of Retinal Rod Bipolar Cells and OFF-Cone Bipolar Subtypes. | ||
| Glia NFkB-signaling promotes glial reactivity and suppresses Müller glia-mediated neuron regeneration in the mammalian retina | ||
| Differentiation Expression patterns of CYP26A1, FGF8, CDKN1A, and NPVF in the developing rhesus monkey retina | ||
| Cell Mol Life Sci Lhx4 surpasses its paralog Lhx3 in promoting the differentiation of spinal V2a interneurons | 


