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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LGR5 Polyclonal antibody

LGR5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30007-1-AP

Synonyms

FEX, G protein coupled receptor 49, G protein coupled receptor 67, GPR49, GPR67, GRP49, HG38, LGR5

Validation Data Gallery

Applications:
  • WB analysis using 30007-1-AP

    WB analysis using 30007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30007-1-AP (LGR5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

30007-1-AP targets LGR5 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGR5 fusion protein Ag31625 相同性解析による交差性が予測される生物種
Full Name leucine-rich repeat-containing G protein-coupled receptor 5
Calculated molecular weight 907 aa, 100 kDa
Observed molecular weight110 kDa
GenBank accession numberBC096324
Gene symbol LGR5
Gene ID (NCBI) 8549
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67) is a protein that in humans is encoded by the LGR5 gene. It is a member of GPCR class A receptor proteins. R-spondin proteins are the biological ligands of LGR5. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.LGR5 is crucial during embryogenesis as LGR null studies in mice incurred 100% neonatal mortality accompanied by several craniofacial distortions such as ankyloglossia and gastrointestinal dilation (PMID: 9642114, 16575208, 525477).

Protocols

Product Specific Protocols
WB protocol for LGR5 antibody 30007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols