LGI3 Polyclonal antibody
LGI3 Polyclonal Antibody for WB, IF, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21919-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, mouse lung tissue, A549 cells, mouse liver tissue, SMMC-7721 cells |
Positive IHC detected in | human lung tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 21919-1-AP is LGI3 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LGI3 fusion protein Ag16257 相同性解析による交差性が予測される生物種 |
Full Name | leucine-rich repeat LGI family, member 3 |
Calculated molecular weight | 548 aa, 62 kDa |
Observed molecular weight | 55-62 kDa |
GenBank accession number | BC142997 |
Gene symbol | LGI3 |
Gene ID (NCBI) | 203190 |
RRID | AB_10838807 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for LGI3 antibody 21919-1-AP | Download protocol |
IHC protocol for LGI3 antibody 21919-1-AP | Download protocol |
IF protocol for LGI3 antibody 21919-1-AP | Download protocol |
FC protocol for LGI3 antibody 21919-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |