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  • Featured Product
  • KD/KO Validated

LDHA-Specific Polyclonal antibody

LDHA-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19987-1-AP

Synonyms

lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, LDHA-Specific, PIG19

Validation Data Gallery

Applications:
  • WB analysis using 19987-1-AP

    WB analysis using 19987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 19987-1-AP

    WB analysis using 19987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using 19987-1-AP

    WB analysis using 19987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 19987-1-AP

    WB analysis of HeLa using 19987-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 19987-1-AP

    WB analysis of mouse testis using 19987-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat kidney using 19987-1-AP

    WB analysis of rat kidney using 19987-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 19987-1-AP

    WB analysis using 19987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 19987-1-AP

    WB analysis of A431 using 19987-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 19987-1-AP

    WB analysis of Jurkat using 19987-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 19987-1-AP

    WB analysis of Jurkat using 19987-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19987-1-AP (LDHA-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse kidney using 19987-1-AP

    IP experiment of mouse kidney using 19987-1-AP

    IP result of anti-LDHA-Specific (IP:19987-1-AP, 4ug; Detection:19987-1-AP 1:2000) with mouse kidney tissue lysate 4000ug.

  • IHC staining of human heart using 19987-1-AP

    IHC staining of human heart using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human heart using 19987-1-AP

    IHC staining of human heart using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human testis using 19987-1-AP

    IHC staining of human testis using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 19987-1-AP

    IHC staining of human testis using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human skeletal muscle using 19987-1-AP

    IHC staining of human skeletal muscle using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skeletal muscle using 19987-1-AP

    IHC staining of human skeletal muscle using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human skeletal muscle using 19987-1-AP

    IHC staining of human skeletal muscle using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 19987-1-AP

    IHC staining of human skeletal muscle using 19987-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 19987-1-AP (LDHA-Specific antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 19987-1-AP

    IF Staining of HepG2 using 19987-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 19987-1-AP

    IF Staining of HepG2 using 19987-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using LDHA-Specific antibody (19987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 19987-1-AP

    FC experiment of HepG2 using 19987-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug LDHA-Specific antibody (19987-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, mouse testis tissue, rat kidney tissue, A431 cells, HeLa cells, NIH/3T3 cells, MCF-7 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

19987-1-AP targets LDHA-Specific in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, goat, chicken, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name lactate dehydrogenase A
Calculated molecular weight 37 kDa
Observed molecular weight 32-37 kDa
GenBank accession numberNM_005566
Gene symbol LDHA
Gene ID (NCBI) 3939
RRIDAB_10646429
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lactate dehydrogenase (LDH) is composed of A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart. The LDHA (lactate dehydrogenase A) and COPB1 (coatomer protein complex, subunit beta 1)genes, are involved in energy metabolism and protein transport processes. Both genes might play important roles in muscle development. It has some isoforms with the molecular mass of 27-40 kDa and can form a homotetramer(PMID:11276087). This antibody is specific to LDHA and has no cross reaction to LDHB and LDHC.

Protocols

Product Specific Protocols
WB protocol for LDHA-Specific antibody 19987-1-APDownload protocol
IHC protocol for LDHA-Specific antibody 19987-1-APDownload protocol
IF protocol for LDHA-Specific antibody 19987-1-APDownload protocol
IP protocol for LDHA-Specific antibody 19987-1-APDownload protocol
FC protocol for LDHA-Specific antibody 19987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

Authors -
View Article
humanWB

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
View Article
humanWB

Drug Resist Updat

Targeting ACYP1-mediated glycolysis reverses lenvatinib resistance and restricts hepatocellular carcinoma progression

Authors - Shuai Wang
  • KD Validated
View Article
human

Nat Immunol

NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.

Authors - Di Huang
View Article
humanWB

Cell Metab

Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.

Authors - Ke Wang
View Article
mouseWB

ACS Nano

Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.

Authors - Mengshi Jiang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Christian (Verified Customer) (01-07-2022)

We expected a huge expression in our primary human chondrocytes and it was perfectly and specifically confirmed by this antibody. The antibody worked also for primary rat chondrocytes. (cell lysis with RIPA-lysis buffer, denaturating conditions)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: human primary chondrocytes
LDHA-Specific Antibody Western Blot validation (1:2000 dilution) in human primary chondrocytes (Cat no:19987-1-AP)