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Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using LAMC1 Polyclonal antibody (20928-1-AP)

    WB analysis of DU 145 using 20928-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 20928-1-AP (LAMC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using LAMC1 Polyclonal antibody (20928-1-AP)

    WB analysis of HeLa using 20928-1-AP

    WB result of LAMC1 antibody (20928-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LAMC1 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using LAMC1 Polyclonal antibody (20928-1-AP)

    WB analysis of HeLa using 20928-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20928-1-AP (LAMC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of DU 145 cells using LAMC1 Polyclonal antibody (20928-1-AP)

    IP experiment of DU 145 using 20928-1-AP

    IP result of anti-LAMC1 (IP:20928-1-AP, 4ug; Detection:20928-1-AP 1:1000) with DU 145 cells lysate 3600ug.

  • Featured Product
  • KD/KO Validated

LAMC1 Polyclonal antibody

LAMC1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 20928-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Laminin subunit gamma 1, LAMB2, LAMC 1, Laminin subunit gamma-1, Laminin-1 subunit gamma

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, HeLa cells
Positive IP detected inDU 145 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20928-1-AP targets LAMC1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14674

Product name: Recombinant human LAMC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1260-1609 aa of BC015586

Sequence: AGDKAVEIYASVAQLSPLDSETLENEANNIKMEAENLEQLIDQKLKDYEDLREDMRGKELEVKNLLEKGKTEQQTADQLLARADAAKALAEEAAKKGRDTLQEANDILNNLKDFDRRVNDNKTAAEEALRKIPAINQTITEANEKTREAQQALGSAAADATEAKNKAHEAERIASAVQKNATSTKAEAERTFAEVTDLDNEVNNMLKQLQEAEKELKRKQDDADQDMMMAGMASQAAQEAEINARKAKNSVTSLLSIINDLLEQLGQLDTVDLNKLNEIEGTLNKAKDEMKVSDLDRKVSDLENEAKKQEAAIMDYNRDIEEIMKDIRNLEDIRKTLPSGCFNTPSIEKP

相同性解析による交差性が予測される生物種
Full Name laminin, gamma 1 (formerly LAMB2)
Calculated molecular weight 178 kDa
Observed molecular weight 220 kDa, 178 kDa
GenBank accession numberBC015586
Gene Symbol LAMC1
Gene ID (NCBI) 3915
RRIDAB_2878767
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP11047
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IP protocol for LAMC1 antibody 20928-1-APDownload protocol
WB protocol for LAMC1 antibody 20928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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