Validation Data Gallery
Tested Applications
| Positive WB detected in | NIH/3T3 cells, Jurkat cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 23 publications below |
| IHC | See 1 publications below |
| IF | See 20 publications below |
Product Information
23498-1-AP targets Laminin beta 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19182 Product name: Recombinant human LAMB1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1437-1786 aa of BC113455 Sequence: AHNAWQKAMDLDQDVLSALAEVEQLSKMVSEAKLRADEAKQSAEDILLKTNATKEKMDKSNEELRNLIKQIRNFLTQDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVESLSQVEVILQHSAADIARAEMLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAIKQADEDIQGTQNLLTSIESETAASEETLFNASQRISELERNVEELKRKAAQNSGEAEYIEKVVYTVKQSAEDVKKTLDGELDEKYKKVENLIAKKTEESADARRKAEMLQNEAKTLLAQANSKLQLLKDLERKYEDNQRYLEDKAQELARLEGEVRSLLKDISQKVAVYSTCL 相同性解析による交差性が予測される生物種 |
| Full Name | laminin, beta 1 |
| Calculated molecular weight | 1786 aa, 198 kDa |
| Observed molecular weight | 220 kDa |
| GenBank accession number | BC113455 |
| Gene Symbol | Laminin beta 1 |
| Gene ID (NCBI) | 3912 |
| RRID | AB_2879288 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07942 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Laminins are a family of basement membrane glycoproteins composed of three nonidentical chains, laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) (PMID: 7959779). Laminins contribute to the structure of extracellular matrix and influence the behavior of associated cells, such as adhesion, differentiation, migration, phenotype stability, and resistance to anoikis (PMID: 23057746). Laminin beta 1 (LAMB1) is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin-1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. Laminin beta 1 is also a subunit of laminin-2 (laminin-211 or merosin), laminin-6 (laminin-311 or K-laminin), laminin-8 (laminin-411), laminin-10 (laminin-511) and laminin-12 (laminin-213).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Laminin beta 1 antibody 23498-1-AP | Download protocol |
| IP protocol for Laminin beta 1 antibody 23498-1-AP | Download protocol |
| WB protocol for Laminin beta 1 antibody 23498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B A novel PGAM5 inhibitor LFHP-1c protects blood-brain barrier integrity in ischemic stroke. | ||
ACS Appl Mater Interfaces Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function. | ||
Cell Rep Stub1 maintains proteostasis of master transcription factors in embryonic stem cells. | ||
J Neuroinflammation SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRβ pathway. | ||
Brain Pathol Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix. | ||
Angiogenesis SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization. |
