Back

Cytokeratin 19 抗体 1 文献

Mouse Monoclonal| カタログ番号: 60187-1-Ig |クローン番号: 3G1E4

注目製品 KD/KO 検証済

  • Print page
  • Download PDF

--

（希望販売価格）

販売および在庫状況はコスモ・バイオ（株）にお問い合わせください。
distributor

適用アプリケーション

アプリケーション:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

Cytokeratin 19 Antibody WB MCF-7 cells 60187-1-Ig

MCF-7 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Cytokeratin 19 Antibody WB HeLa cells 60187-1-Ig

WB result of KRT19 antibody (60187-1-IG, 1:10,000) with si-Control and si-KRT19 transfected HeLa cells.

Cytokeratin 19 Antibody IHC human colon tissue 60187-1-Ig

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cytokeratin 19 Antibody IHC human colon tissue 60187-1-Ig

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cytokeratin 19 Antibody IF human breast cancer tissue 60187-1-Ig

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Cytokeratin 19 antibody (60187-1-Ig, Clone: 3G1E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

種特異性:
human
WB ポジティブ:
MCF-7 cells, HeLa cells
IHC ポジティブ:
human colon tissue, human lung cancer tissue, human stomach cancer tissue, human breast cancer tissue, human thyroid cancer tissue
IF ポジティブ:
human breast cancer tissue
推奨希釈倍率:
WB : 1:500-1:2000
IHC : 1:1000-1:51200
IF : 1:200-1:800

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein A purification
アイソタイプ:
IgG2b
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
Cytokeratin 19 fusion protein Ag7407
正式名:
keratin 19
計算分子量:
44 kDa
検出分子量:
48 kDa
GenBank accession number:
BC002539
Gene ID (NCBI):
3880
遺伝子シンボル:
KRT19
別名:
CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19

背景説明

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma.


Back
to top